SRP0113
HDAC-11 human
recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)
Synonym(s):
HD11, Histone deacetylase 11
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥10% (SDS-PAGE)
form
aqueous solution
mol wt
66 kDa
packaging
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HDAC11(79885)
General description
HDAC-11 (histone deacetylase 11) is a zinc-dependent HDAC. It belongs to the HDAC family of proteins and HDAC class IV. It is highly conserved among various species. The mRNA is present in the brain, skeletal muscle, kidney and testis. The gene is mapped to human chromosome 3p25.
Human HDAC11, full length, GenBank Accession No. BC009676, with N-terminal GST tag, MW = 66kDa, expressed in baculovirus expression system.
Human HDAC11, full length, GenBank Accession No. BC009676, with N-terminal GST tag, MW = 66kDa, expressed in baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
HDAC-11 (histone deacetylase 11) is responsible for deacetylation of nuclear histone and nonhistone proteins, including transcription factors. HDAC-11 catalyzes the deacetylation of histone H4. It binds to CDT1 (DNA replication factor) and is present with S phase-associated replication origins. HDAC-11 is upregulated in various carcinomas. Absence of it results in cell death and suppresses metabolic activity in colon, prostate, breast and ovarian cancer cell lines.
Unit Definition
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma.
Buglio D, et al.
Blood, 117, 2910-2910 (2011)
Chromosomal organization and localization of the novel class IV human histone deacetylase 11 gene.
Voelter-Mahlknecht S, et al.
International Journal of Molecular Medicine, 16, 589-589 (2005)
Chromatin unfolding by Cdt1 regulates MCM loading via opposing functions of HBO1 and HDAC11-geminin.
Wong PG, et al.
Cell Cycle, 9, 4351-4351 (2010)
HDAC11 is a novel drug target in carcinomas.
Deubzer HE, et al.
International Journal of Cancer. Journal International Du Cancer, 132, 2200-2200 (2013)
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