SRP0103
HDAC2 FLAG tag human
recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)
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Synonym(s):
HD2, Histone deacetylase 2, Transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥50% (SDS-PAGE)
form
aqueous solution
mol wt
60 kDa
packaging
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... HDAC2(3066)
General description
Human HDAC2, GenBank Accession No. Q92769, full length with C-terminal DDDDK tag (FLAG), MW = 60 kDa, expressed in baculovirus expression system.
The HDAC2 (histone deacetylase 2) gene is mapped to human chromosome 6q21 and is ubiquitously expressed.
The HDAC2 (histone deacetylase 2) gene is mapped to human chromosome 6q21 and is ubiquitously expressed.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Histone deacetylase 2 (HDAC2) is known to regulate DNA damage signalling, maintain genomic stability and prevents tumor formation and progression. Hdac2 does not possess a DNA binding domain, it forms multi-component repressor complexes for its interaction with transcription factors. Hdac2 also mediates cell proliferation, differentiation and apoptosis. HDAC2 controls response to chronic inflammation in COPD (chronic obstructive pulmonary disease). It activates Wnt signaling pathway via β-catenin T-cell transcription factor. Deletion of HDAC2 along with HDAC1 leads to anemia and thrombocytopenia.
Unit Definition
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
CRISPR-mediated HDAC2 disruption identifies two distinct classes of target genes in human cells.
Somanath P, et al.
PLoS ONE, 12(10), e0185627-e0185627 (2017)
Transcription and beyond: the role of mammalian class I lysine deacetylases.
Moser M A, et al.
Chromosoma, 123(1-2), 67-78 (2014)
Epigenetic changes in diabetes.
Al-Haddad R, et al.
Neuroscience Letters, 625, 64-69 (2016)
HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130.
Goder A, et al.
Nature Communications, 9(1), 764-764 (2018)
Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.
Ma P and Schultz R M
PLoS Genetics, 9(3), e1003377-e1003377 (2013)
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