SRE0119
dsDNase
For gDNA removal, recombinant, expressed in Pichia pastoris, Heat labile, Triton-free
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Synonym(s):
HL-dsDNase, heat labile double strand specific deoxyribonuclease
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
recombinant
expressed in Pichia pastoris
Quality Level
composition
Triton-free
packaging
pkg of 250 U
shipped in
dry ice
storage temp.
−20°C
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General description
Double-strand specific DNases (dsDNases) are unique double-strand specific endonucleases. As they do not digest ssDNA or RNA, they can be used to specifically remove dsDNA in the presence of other nucleic acids.
Application
This heat labile dsDNase removes genomic DNA from RNA preparations with minimal impact on RNA integrity.with minimal impact on RNA integrity
Features and Benefits
Due to the mild inactivation temperature required, this dsDNase can remove genomic DNA from RNA perparations and have minimal impact onto RNA quality and quantity.
Unit Definition
One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
Reconstitution
In solution
Other Notes
The heat labile dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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