SRE0119
dsDNase
For gDNA removal, recombinant, expressed in Pichia pastoris, Heat labile, Triton-free
Synonym(s):
HL-dsDNase, heat labile double strand specific deoxyribonuclease
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352204
Recombinant:
expressed in Pichia pastoris
recombinant
expressed in Pichia pastoris
composition
Triton-free
packaging
pkg of 250 U
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
Double-strand specific DNases (dsDNases) are unique double-strand specific endonucleases. As they do not digest ssDNA or RNA, they can be used to specifically remove dsDNA in the presence of other nucleic acids.
Application
This heat labile dsDNase removes genomic DNA from RNA preparations with minimal impact on RNA integrity.with minimal impact on RNA integrity
Features and Benefits
Due to the mild inactivation temperature required, this dsDNase can remove genomic DNA from RNA perparations and have minimal impact onto RNA quality and quantity.
Preparation Note
In solution
Other Notes
One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
The heat labile dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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