SRE0117
Salt Active Nuclease (SAN)
High Purity, recombinant, expressed in Pichia pastoris, Triton-free
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Synonym(s):
endonuclease, salt active endonuclease, salt tolerant nuclease
UNSPSC Code:
12352200
NACRES:
NA.54
Recommended Products
recombinant
expressed in Pichia pastoris
Quality Level
composition
Triton-free
packaging
pkg of 25 kU
shipped in
dry ice
storage temp.
−20°C
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General description
This Salt Active Nuclease (SAN) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
Application
- Useful for purification of tagged recombinant proteins
- Manufacturing of nucleic acid-free molecular enzymes and reagents
- Research of vaccines, viruses and antibodies
Features and Benefits
- Active at low temperatures
- Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
- Typically does not bind to anion exchange or IMAC media
- Detergent free No protease detected
Unit Definition
One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCI pH 8.5 (@25 °C), 5 mM MgCl2, 500 mM NaCl, and 50 µg/mL calf thymus DNA.
Reconstitution
In solution
Other Notes
- Temperature: 5 – 40°C, optimal: ~35°C
- Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
- Mg2+: >1 mM is required for activity, optimal 5 - 50 mM
- pH: 7.3 – 10.0, optimal 8.2 - 9.2
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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