SRE0112
Heat-labile Cod Uracil-DNA Glycosylase
recombinant, expressed in E. coli, Triton-free and Glycerol-free
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Synonym(s):
UDG, UNG
UNSPSC Code:
41106300
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
composition
Triton-free and Glycerol-free
shipped in
dry ice
storage temp.
−20°C
General description
Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. UracilDNA Glycosylase, also known as UNG or UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E.coli (ung–) strain that contains a modified Cod UNG gene.
Specificity
One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37°C in 70 mm Tris-HCI pH 8.0 (@25°C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.
Application
Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP
Features and Benefits
- Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
- Cod UNG is not active on uracil in RNA.
- High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
- Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction
Reconstitution
In solution
Other Notes
This product is for R&D use only. Not for drug, household, or other uses.
related product
Product No.
Description
Pricing
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Uracil-DNA glycosylases?structural and functional perspectives on an essential family of DNA repair enzymes
Schormann N, et al.
Protein Science, 23(12), 1667-1685 (2014)
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