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CN

SRE0082

Peroxidase from horseradish

For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US, Type VI, powder, stabilized, ≥250 units/mg solid (using pyrogallol)

Synonym(s):

Peroxidase from horseradish, horse radish peroxidase, horseradish peroxidase, horseradish peroxidase (hrp), hrp, peroxidase, peroxidase enzyme, peroxidase from horseradish, Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

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About This Item

CAS Number:
9003-99-0
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-668-6
MDL number:
MFCD00071339
EC Number:
1.11.1.7 (BRENDA, IUBMB)

Key Documents

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Product Name

Peroxidase from horseradish, For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US, Type VI, powder, stabilized, ≥250 units/mg solid (using pyrogallol)

SMILES string

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

type

Type VI

form

powder, stabilized

specific activity

≥250 units/mg solid (using pyrogallol)

technique(s)

ELISA: suitable
immunoassay: suitable
lateral flow assay: suitable
western blot: suitable

color

brown

solubility

phosphate buffer: soluble

suitability

suitable for immunoassay
suitable for manufacturing use

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

2-8°C

Quality Level

400

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Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Application

HRP is used in immunoassays, such as ELISAs, Western blots, and lateral flow assays. HRP is used in academic research, diagnostics, and pharmaceutical research. For more applications, see the following technical article: An Introduction to Horseradish Peroxidase (HRP) and Its Applications.
Peroxidase from horseradish has been used in western blotting.

General description

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). HRP is a single chain polypeptide containing four disulfide bonds. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes is 3.0 - 9.0.
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Other Notes

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

Regulatory Information

动植物源性产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000465782
0000465781
0000465782
0000465781
0000417952

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Yonghao Sun et al.
Experimental and therapeutic medicine, 14(2), 1809-1817 (2017-08-16)
The aim of the present study was to investigate the inhibitory effect of compound cantharides capsules (CCCs) on the viability and apoptosis of human gastric cancer cell lines, BGC-823 and SGC-7901, and to detect its regulation of gene expression levels
Roles of p38 and JNK protein kinase pathways activated by compound cantharidin capsules containing serum on proliferation inhibition and apoptosis of human gastric cancer cell line
Sun Y, et al.
Experimental and Therapeutic Medicine, 14(2), 1809-1817 (2017)
Leilei Bao et al.
Scientific reports, 12(1), 10693-10693 (2022-07-02)
Infectious diseases are among the leading causes of mortality worldwide. A new coronavirus named severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) was identified in Wuhan, China in 2019, and the World Health Organization (WHO) declared its outbreak, coronavirus disease

Articles

An Introduction to Horseradish Peroxidase (HRP) and Its Applications

Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.

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