Skip to Content
Merck
CN
All Photos(2)

Documents

Safety Information

SRE0069

Sigma-Aldrich

Phosphoglucomutase from rabbit muscle

Synonym(s):

α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

CAS Number:
9001-81-4
Enzyme Commission number:
5.4.2.2 (BRENDA, IUBMB)
MDL number:
MFCD00131886
UNSPSC Code:
12352204
NACRES:
NA.32

form

lyophilized powder

Quality Level

200

specific activity

≥100 units/mg protein

foreign activity

Lactic dehydroenase ≤0.5%
Phosphoglucose Isomerase ≤0.01%
pyruvate kinase ≤0.05%

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Research area: CellSignalling. The phosphoglucomutase (PGM) molecule, a single polypeptide chain, is made up of four α/β domains. The domains form a compact heart-shaped structure with a large fissure between the two lobes, and the molecule comprises about 40 secondary structural components. The active enzymatic site of the molecule is located at the bottom of the crevice on the surface of domain I. Rabbit muscle phosphoglucomutase (RB-PGM) is a monomer with a unique four-domain architecture.

Application

Phosphoglucomutase from rabbit muscle may be used in dephosphorylation to investigate the hexose bisphosphate activation of phosphoglucomutase.

Biochem/physiol Actions

Phosphoglucomutase (PGM) mainly catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate via a glucose 1,6-diphosphate intermediate. Hence it plays a key role in glycolysis and gluconeogenesis.

Unit Definition

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Physical form

Lyophilized powder containing Tris HCl, EDTA, magnesium acetate and carbohydrate

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物源性产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

C M Galloway et al.
Plant physiology, 79(3), 920-922 (1985-11-01)
The hexose bisphosphate activation of phosphoglucomutase was investigated with both plant (pea and mung bean) and animal (rabbit muscle) sources of the enzyme. Plant phosphoglucomutase was purified about 50-fold from seeds, and to a lesser extent, from seedlings of Pisum
S Levin et al.
Protein engineering, 12(9), 737-746 (1999-10-03)
Three-dimensional structural models of three members of the phosphoglucomutase (PGM) superfamily, parafusin, phosphoglucomutase-related protein and sarcoplasmic reticulum phosphoglucomutase, were constructed by homology modeling based on the known crystal structure of rabbit muscle phosphoglucomutase. Parafusin, phosphoglucomutase-related protein and sarcoplasmic reticulum phosphoglucomutase

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service