SRE0001
Pepsin from porcine gastric mucosa
Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein
Synonym(s):
Pepsin A, Pepsin from hog stomach
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-629-3
MDL number:
Specific activity:
≥3200 units/mg protein
Quality Level
form
lyophilized powder
specific activity
≥3200 units/mg protein
mol wt
35 kDa
impurities
salt, essentially free
color
white to off-white
UniProt accession no.
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
Gene Information
pig ... LOC396892(396892)
Application
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
Biochem/physiol Actions
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Protein determined by E1%/280
Other Notes
One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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Tina L Samuels et al.
The Annals of otology, rhinology, and laryngology, 119(3), 203-208 (2010-04-16)
Diagnosis of extraesophageal reflux (EER) currently relies on tools designed for diagnosis of gastroesophageal reflux. Such tools lack the sensitivity and reproducibility to detect the less frequent and mildly acidic reflux associated with upper airway disease. Pepsin has been posited
Adrian Allen et al.
American journal of physiology. Cell physiology, 288(1), C1-19 (2004-12-14)
Secretion of bicarbonate into the adherent layer of mucus gel creates a pH gradient with a near-neutral pH at the epithelial surfaces in stomach and duodenum, providing the first line of mucosal protection against luminal acid. The continuous adherent mucus
Yi Sun et al.
Journal of natural products, 77(7), 1749-1752 (2014-06-25)
Two linear peptides, ahpatinin Ac (1) and ahpatinin Pr (2), were isolated together with the known ahpatinin (i)Bu, pepstatin Ac, pepstatin Pr, and pepsinostreptin from a Streptomyces sp. derived from a deep-sea sediment. The structure of ahpatinin Pr (2) was
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SRE0001-10G | 04061833343418 |
| SRE0001-5G | 04061833343425 |
| SRE0001-100G | 04061837108822 |