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SML3797

Sigma-Aldrich

Biotin-HPDP

≥95% (HPLC)

Synonym(s):

(3aS,4S,6aR)-Hexahydro-2-oxo-N-[6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexyl]-1H-thieno[3,4-d]imidazole-4-pentanamide, HPDP-Biotin, N-[6-(Biotinamido)hexyl]-3′-(2′-pyridyldithio)propionamide

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About This Item

Empirical Formula (Hill Notation):
C24H37N5O3S3
CAS Number:
129179-83-5
Molecular Weight:
539.78
MDL number:
MFCD00078541
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥95% (HPLC)

form

powder

storage condition

protect from light

color

white to beige

solubility

DMSO: 2 mg/mL, clear (Warmed)

storage temp.

-10 to -25°C

Biochem/physiol Actions

Biotin-HPDP (HPDP-Biotin) is a membrane-permeable and reversible biotinylating reagent that is used to biotinylate target proteins via covalent disulfide bond (-S-S-) formation with free sulfhydryl (-SH) groups. The biotin-tagged proteins can then be subjected to affinity labeling or isolation/purification using avidin conjugates or beads. Unlike tranditonal biotinylation methods, Biotin-HPDP tag can be removed with reducing agents such as dithiothreitol (DTT) without the need of harsh reagents to dissociate the high affinity interaction between avidin and biotin.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Low-Background Acyl-Biotinyl Exchange Largely Eliminates the Coisolation of Non- S-Acylated Proteins and Enables Deep S-Acylproteomic Analysis
Analytical Chemistry, 91(15), 9858-9866 (2019)
Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins
Free Radical Biology & Medicine, 44(7), 1362-1372 (2008)
Localization and partial characterization of the oligomeric disulfide-linked molecular weight 95,000 protein (triadin) which binds the ryanodine and dihydropyridine receptors in skeletal muscle triadic vesicles
Biochemistry, 30(30), 7507-7513 (1991)

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