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SHC332V
MISSION® 3X LacO Inducible Non-Target shRNA Control Transduction Particles
Does not target any known genes
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CN¥7,012.18
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About This Item
CN¥7,012.18
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This Item | M5310 | M3516 | M1180 |
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) cell culture | embryo: suitable | technique(s) PCR: suitable | technique(s) - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| grade for molecular biology | grade - | grade - | grade - |
| form viscous liquid | form light oil | form light oil | form liquid |
| cation traces Ca: ≤10 mg/kg, Co: ≤5 mg/kg, Cu: ≤5 mg/kg, K: ≤50 mg/kg, Mn: ≤5 mg/kg, Ni: ≤5 mg/kg, Zn: ≤5 mg/kg, Cd: ≤5 mg/kg, Fe: ≤5 mg/kg, Na: ≤50 mg/kg, Cr: ≤5 mg/kg, Pb: ≤5 mg/kg, Mg: ≤5 mg/kg | cation traces - | cation traces - | cation traces - |
| UV absorption λ: 260 nm Amax: 0.7, λ: 280 nm Amax: 1.0 | UV absorption - | UV absorption - | UV absorption - |
General description
The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA.
The MISSION 3x LacO Inducible Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. 200 uL of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.
Application
Legal Information
recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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