SHC204
MISSION® TRC2 pLKO.5-puro TurboGFP™ shRNA Control Plasmid DNA
shRNA sequence targeting tGFP
Synonym(s):
MISSION® Control Vectors
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About This Item
Quality Level
100
200
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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General description
The MISSION® TRC2 Control Vector pLKO-puro TurboGFP™ shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP™. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP™ shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION® TurboGFP™ positive control vector in cell lines expressing TurboGFP™. TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP™ shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP™ shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
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Regulatory Information
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