SHC015
MISSION® pLKO.1-puro-UbC-TagFP635™ Positive Control Plasmid DNA
Contains a gene encoding TagFP635, under the control of the UbC promoter
Synonym(s):
MISSION® Control Vectors
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About This Item
MDL number:
UNSPSC Code:
12352200
product line
MISSION®
shipped in
dry ice
storage temp.
−20°C
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General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TagFP635 Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Vector is a 9226 base pair lentivirus plasmid vector that contains a gene encoding TagFP635, under the control of the UbC promoter. The UbC-TagFP635 Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Vector is a 9226 base pair lentivirus plasmid vector that contains a gene encoding TagFP635, under the control of the UbC promoter. The UbC-TagFP635 Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagFP635 is a trademark of Evrogen Co.
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