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Safety Information

SH2711

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock

JAK-STAT Pathway

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500 G
¥1,018.94
2.5 KG
¥3,275.29

¥1,018.94

Estimated to ship on2025年7月01日Details

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500 G
¥1,018.94
2.5 KG
¥3,275.29

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

¥1,018.94

Estimated to ship on2025年7月01日Details

Request a Bulk OrderRequest more information

Quality Level

200

product line

MISSION®

storage temp.

−70°C

Gene Information

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1 of 4

This Item
S9627S564013464
硫酸钠 BioXtra, ≥99.0%

S6547

硫酸钠

硫酸钠 ReagentPlus®, ≥99.0%

S9627

硫酸钠

硫酸钠 ≥99.0%, suitable for plant cell culture

S5640

硫酸钠

硫酸钠 puriss., meets analytical specification of Ph. Eur., BP, USP, anhydrous, 99.0-100.5% (calc. to the dried substance)

13464

硫酸钠

form

powder

form

powder

form

powder

form

crystalline

assay

≥99.0%

assay

≥99.0%

assay

≥99.0%

assay

99.0-100.5% (calc. to the dried substance)

solubility

H2O: 1 M, clear, colorless

solubility

-

solubility

water: soluble 1 g/10 mL

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

density

2.68 g/mL at 25 °C (lit.)

density

2.68 g/mL at 25 °C (lit.)

density

2.68 g/mL at 25 °C (lit.)

density

2.68 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.0005%, Cu: ≤0.0005%, K: ≤0.005%, NH4+: ≤0.05%, Zn: ≤0.001%, Ca: ≤0.005%, Mg: ≤0.005%, Fe: ≤0.0005%, Pb: ≤0.001%

cation traces

-

cation traces

-

cation traces

As: ≤2 mg/kg, Ca: ≤50 mg/kg, Fe: ≤50 mg/kg, Mg: ≤50 mg/kg, Zn: ≤300 mg/kg

General description

The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as 50 μl bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 15% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 190, Number of Clones: 1358
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Peter J Murray
    Journal of immunology (Baltimore, Md. : 1950), 178(5), 2623-2629 (2007-02-22)
    Universal and essential to cytokine receptor signaling, the JAK-STAT pathway is one of the best understood signal transduction cascades. Almost 40 cytokine receptors signal through combinations of four JAK and seven STAT family members, suggesting commonality across the JAK-STAT signaling
    Lynda A O'Sullivan et al.
    Molecular immunology, 44(10), 2497-2506 (2007-01-09)
    The complexity of multicellular organisms is dependent on systems enabling cells to respond to specific stimuli. Cytokines and their receptors are one such system, whose perturbation can lead to a variety of disease states. This review represents an overview of

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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