SH1321
MISSION® shRNA Human Gene Family Set, DNA
Cytokine and Chemokine Receptors
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About This Item
UNSPSC Code:
41106609
NACRES:
NA.51
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General description
Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.
Other Notes
Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 93, Number of Clones: 584
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Samantha J Allen et al.
Annual review of immunology, 25, 787-820 (2007-02-13)
Chemokines are critical mediators of cell migration during routine immune surveillance, inflammation, and development. Chemokines bind to G protein-coupled receptors and cause conformational changes that trigger intracellular signaling pathways involved in cell movement and activation. Although chemokines evolved to benefit
Céline Callewaere et al.
Journal of molecular endocrinology, 38(3), 355-363 (2007-03-07)
Chemokines are small secreted proteins that chemoattract and activate immune and non-immune cells both in vivo and in vitro. In addition to their well-established role in the immune system, several recent reports have suggested that chemokines and their receptors may
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