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Safety Information

SH1311

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock

Cytokine and Chemokine Receptors

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500 MG
¥527.00

¥527.00

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500 MG
¥527.00

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

¥527.00

Please contact Customer Service for Availability
Request a Bulk OrderRequest more information

Quality Level

200

product line

MISSION®

storage temp.

−70°C

Gene Information

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胆影葡胺 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1324

胆影葡胺

麦芽糖 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1497

麦芽糖

麦芽三糖 Pharmaceutical Secondary Standard; Certified Reference Material

PHR2086

麦芽三糖

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical small molecule

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

format

neat

format

neat

format

neat

format

neat

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

storage temp.

2-8°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

General description

The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as 50 μl bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 15% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 93, Number of Clones: 584
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--病毒，疫苗等

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    Samantha J Allen et al.
    Annual review of immunology, 25, 787-820 (2007-02-13)
    Chemokines are critical mediators of cell migration during routine immune surveillance, inflammation, and development. Chemokines bind to G protein-coupled receptors and cause conformational changes that trigger intracellular signaling pathways involved in cell movement and activation. Although chemokines evolved to benefit
    Céline Callewaere et al.
    Journal of molecular endocrinology, 38(3), 355-363 (2007-03-07)
    Chemokines are small secreted proteins that chemoattract and activate immune and non-immune cells both in vivo and in vitro. In addition to their well-established role in the immune system, several recent reports have suggested that chemokines and their receptors may
    The many roles of chemokines and chemokine receptors in inflammation.
    Israel F Charo et al.
    The New England journal of medicine, 354(6), 610-621 (2006-02-10)

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