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Safety Information

SEP040

Millipore

Seppro® IgY14

LC10

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

column D × L

12.7 mm × 79.0 mm

capacity

12.5 mg total protein loading(178 μL of human plasma, average human protein conc 70 mg/mL)

storage temp.

2-8°C

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General description

Seppro® IgY14 columns allow users to separate 14 highly abundant proteins (HAP) from plasma protein mixtures. The Avian polyclonal IgY (Immunoglobulin Yolk) antibodies, have a distinct, highly specific partitioning advantage over competing products, allowing the researcher to look at a much cleaner human protein mixture. Seppro® columns make further proteomics analysis into the plasma proteome much easier and provide cleaner results.

Proteins Depleted:

Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Complement C3
α1-Acid Glycoprotein (Orosomucoid)
HDL (Apolipoproteins A-I and A-II)
LDL (mainly Apolipoprotein B)

Application

Seppro® IgY14, LC10 (10mL bed volume columns) can be used as a plasma/serum protein removal tool base upon immune affinity removal of 14 highly abundant proteins (HAP) from human plasma protein mixtures via liquid chromatography. Seppro® IgY14 is used to support the analysis of low abundance human serum/plasma proteins.

Legal Information

Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • S4199Seppro® Dilution bufferSDS
  • S4324Seppro® Stripping BufferSDS
  • S4449Seppro® Neutralization BufferSDS
  • CLS8163Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterileSDS

Storage Class Code

10 - Combustible liquids

Regulatory Information

新产品

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Certificates of Analysis (COA)

Lot/Batch Number
SLCB6637

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. My protein sample contains SDS.  Is this compatible with Product SEP040, Seppro® IgY14?  

    No. We do not recommend using protein samples containing SDS with Product No. SEP040. SDS is a detergent and would be expected to affect adversely the activity of the conjugated antibody in terms of depletion.

  7. How would I decide between Products SEP040, Seppro® IgY14 and PROT20LC, ProteoPrep® 20 Plasma Immunodepletion LC Columns?

    SEP040 is designed to deplete the top 14 abundant human proteins, while PROT20LC is designed to deplete the top 20 abundant human proteins. Also, all of the antibodies in SEP040 are IgY antibodies, while the PROT20LC antibodies and antibody ligands are primarily IgG-based.

  8. I originally purchased my Seppro IgY14 LC10 column from Genway.  Can I still contact Genway for technical support?

    All inquiries on the Seppro technology are now to be referred to Sigma-Aldrich. Customers should not contact Genway for technical assistance.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nicole Haverland et al.
Journal of translational medicine, 8, 137-137 (2010-12-22)
Proteomic-based discovery of biomarkers for disease has recently come under scrutiny for a variety of issues; one prominent issue is the lack of orthogonal validation for biomarkers following discovery. Validation by ELISA or Western blot requires the use of antibodies
Tujin Shi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(38), 15395-15400 (2012-09-06)
Sensitive detection of low-abundance proteins in complex biological samples has typically been achieved by immunoassays that use antibodies specific to target proteins; however, de novo development of antibodies is associated with high costs, long development lead times, and high failure
Yuan-Wan Sun et al.
Journal of proteome research, 18(9), 3461-3469 (2019-08-02)
We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between
Chien-Lun Chen et al.
Journal of proteomics, 85, 28-43 (2013-05-02)
In this study, we evaluated the reproducibility of abundant urine protein depletion by hexapeptide-based library beads and an antibody-based affinity column using the iTRAQ technique. The antibody-based affinity-depletion approach, which proved superior, was then applied in conjunction with iTRAQ to
Majlinda Kullolli et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 939, 10-16 (2013-10-05)
Human plasma is a commonly used diagnostic fluid in clinical chemistry. In-depth plasma proteomic analysis is performed to search for disease biomarkers, however the large dynamic range of protein abundance in plasma presents a substantial analytical challenge. Removal of abundant
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