SCA20
Sucrose Assay Kit
sufficient for 20 assays
Synonym(s):
Sucrose Test Kit
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About This Item
UNSPSC Code:
12164500
EC Number:
200-334-9
NACRES:
NA.84
Product Name
Sucrose Assay Kit, sufficient for 20 assays
Quality Level
usage
sufficient for 20 assays
analyte chemical class(es)
sugars (sucrose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.
General description
Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.
Storage Class
10 - Combustible liquids
Regulatory Information
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Bergmeyer, H.U. and Bernt, E.
Methods of Enzymatic Analysis, 1176-1176 (1974)
Ye Ji Lee et al.
Frontiers in plant science, 12, 644455-644455 (2021-07-20)
Nitrogen is one of the most important macro-nutrients for plant growth and crop productivity. The amount of synthetic nitrogen fertilizers supplied to crops has dramatically increased, leading to a notable rise in crop yields. However, excessive nitrogen use has an
Ching Man Wai et al.
BMC genomics, 18(1), 773-773 (2017-10-13)
Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014
Manish Pandey et al.
Scientific reports, 11(1), 3000-3000 (2021-02-06)
Plant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were assessed
Raja S Payyavula et al.
BMC plant biology, 12, 39-39 (2012-03-21)
Plant secondary metabolites, including phenylpropanoids and carotenoids, are stress inducible, have important roles in potato physiology and influence the nutritional value of potatoes. The type and magnitude of environmental effects on tuber phytonutrients is unclear, especially under modern agricultural management
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