SBPE05
SB-BCRP-M-PREDEASY-ATPase kit
membrane based kit for Human BCRP ATPase Transport Assays
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Synonym(s):
ABCG2, BCRP, BCRP human membrane based kit for Human BCRP ATPase Transport Assays, Breast Cancer Resistance Protein
UNSPSC Code:
12161503
Recommended Products
recombinant
expressed in human cells
expressed in insect cells
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ABCG2(9429)
General description
SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY™ ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.
Application
SOLVO′s PREDEASY™ ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.
PREDIVEZ™ PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.
PREDIVEZ™ PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.
Physical form
Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.
Legal Information
Distributed for SOLVO Biotechnology, Inc.
PREDEASY is a trademark of Solvo Biotechnology
PREDIVEZ is a trademark of Solvo Biotechnology
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
131.0 °F
Flash Point(C)
55 °C
Regulatory Information
新产品
Certificates of Analysis (COA)
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Márton Jani et al.
Journal of pharmaceutical sciences, 100(1), 94-97 (2010-06-25)
Ivermectin is an antiparasitic drug frequently administered to humans. It has a limited brain exposure that is attributed to the efflux activity of ABCB1/Abcb1. ABCG2/Abcg2 is also a major transporter present in most pharmacologically important barriers. However, interaction of ivermectin
Albert Mennone et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(10), 1673-1678 (2010-07-06)
Breast cancer resistance protein (Bcrp) is a member of the ATP-binding cassette membrane transporter family, which is expressed apically in liver, kidney, and intestine epithelium. Recent reports suggest that in addition to xenobiotics, porphyrins, and food toxins, Bcrp can also
Márton Jani et al.
Biological & pharmaceutical bulletin, 32(3), 497-499 (2009-03-03)
The pharmacokinetics of sulfasalazine, an anti-inflammatory drug is influenced by ATP-binding cassette G2 (ABCG2) (breast cancer resistance protein (BCRP), mitoxantrone resistance protein (MXR)) both in vitro and clinically. Due to its low passive permeability, the intracellular concentration of sulfasalazine is
E Kis et al.
Annals of the rheumatic diseases, 68(7), 1201-1207 (2008-04-10)
Earlier publications have suggested a possible role for the efflux transporter breast cancer resistance protein (BCRP) in acquired resistance to disease-modifying antirheumatic drugs (DMARDs) such as leflunomide and its metabolite A771726 (teriflunomide). However, there is no direct evidence that BCRP
M Maliepaard et al.
Cancer research, 61(8), 3458-3464 (2001-04-20)
High expression of the Breast Cancer Resistance Protein (BCRP) gene has been shown to be involved in resistance to chemotherapeutic drugs. Knowledge of the localization of BCRP protein in normal tissues may help unravel the normal function of this protein.
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