SAE0212
Mucin Probe, StcE
Biotin and FLAG® tagged, powder
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Synonym(s):
Catalytically inactive Mucin specific protease StcE
NACRES:
NA.77
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General description
Mucin Probe, StcE is a proteolytically inactive mutant (E447D) form of Mucinase StcE that maintains ability to tightly bind mucin proteins. The recombinant protein carries a 3XFLAG tag and is also enzymatically biotinylated at the C-term of the protein. While there are many applications, this product is designed to probe mucin dense tissues. It will non-covalently, and selectively bind to Mucin-domain glycoproteins with dense O-linked glycosylation. Secondary reporting tools can then be used to bind the probe using its FLAG® tag or Biotin conjugate.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Regulatory Information
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Keira E Mahoney et al.
Current protocols, 4(7), e1100-e1100 (2024-07-10)
Mucin-domain glycoproteins are characterized by their high density of glycosylated serine and threonine residues, which complicates their analysis by mass spectrometry. The dense glycosylation renders the protein backbone inaccessible to workhorse proteases like trypsin, the vast heterogeneity of glycosylation often
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