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Safety Information

SAE0069

Sigma-Aldrich

Cholera Toxin B subunit

recombinant, expressed in HEK 293 cells, subunit A free

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Synonym(s):
CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB
UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in HEK 293 cells

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

quality

(The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.)

specific activity

(Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)

mol wt

pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing) (Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.)
calculated mol wt 13 kDa
observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)

solubility

H2O: soluble 11 mg/mL

UniProt accession no.

P01556

storage temp.

−20°C

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

Application

Cholera Toxin B subunit has been used:
  • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
  • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--毒素类产品

Certificates of Analysis (COA)

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Si-Yuan Song et al.
Frontiers in neuroanatomy, 14, 600555-600555 (2020-12-18)
This study aimed to investigate the direct monosynaptic projections from cortical functional regions to the cerebrospinal fluid (CSF)-contacting nucleus for understanding the functions of the CSF-contacting nucleus. The Sprague-Dawley rats received cholera toxin B subunit (CB) injections into the CSF-contacting

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