SAE0023
Alanine Racemase from Bacillus stearothermophilus
buffered aqueous solution, ≥10 IU/mg solid
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Synonym(s):
Alanine Racemase from Bacillus stearothermophilus
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
Bacillus sp. (B. stearothermophilus)
Quality Level
form
buffered aqueous solution
specific activity
≥10 IU/mg solid
mol wt
Mr 78 kDa (2 subunits 39 kDa each)
storage temp.
−20°C
Application
Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.
Biochem/physiol Actions
Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†
Unit Definition
One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.
Physical form
Aqueous solution containing 10 mM potassium phosphate
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
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