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SAB5801015

Sigma-Aldrich

Alpaca Anti-Mouse kappa single domain Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(s):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

alpaca

recombinant

expressed in E. coli

conjugate

unconjugated (6*his tag and one cys were added at the C terminal of the VHH)

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

recombinant monoclonal

form

lyophilized solid

mol wt

14 kDa

species reactivity

mouse, Alpaca

technique(s)

ELISA: 1:5000-1:20000
western blot: 1:5000-1:20000

size

0.1 mL

color

colorless

isotype

VHH

shipped in

wet ice

storage temp.

2-8°C

General description

Alpaca Anti-Mouse kappa single domain Antibody is designed to specifically detect mouse IgG kappa chain. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse kappa single domain Antibody reacts with the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Other Notes

No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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