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SAB5202071

Sigma-Aldrich

Monoclonal Anti-beta 4 Spectrin-Horseradish Peroxidase antibody produced in mouse

clone S393-2, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SPTBN4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.45

biological source

mouse

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

S393-2
monoclonal

description

clone S393-2, purified immunoglobulin, buffered aqueous solution
target molecular weight: 200 kDa

form

buffered aqueous solution

species reactivity

human (Tested), rat (Predicted), African green monkey (Tested), mouse (Predicted)

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles
protect from light

concentration

1.0 mg/mL

technique(s)

immunofluorescence: 1:100
western blot: 1:1000

isotype

IgG1

UniProt accession no.

Q9H254

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SPTBN4(57731)

Immunogen

Immunogen Description: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
Immunogen Region: C-terminal
Immunogen Species: Human
Immunogen type: Fusion protein

Application


Western blotting
Dilution 1:1000
Tissue/Cell Type Used: monkey COS-Beta-4-Spectrin-His

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: SK-N-BE

Physical form

50% glycerol, 0.1% sodium azide
PBS pH 7.4

Preparation Note

Derived from animal serum

Storage and Stability

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Other Notes

Additional Product Details:
Does not cross react with other Beta-spectrins
Lot Expiration/Retest Date: Expires 1 year from purchase
Target/Gene Description: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart . Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier .
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_066022.2

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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