Anti-SARS-COV-2-Spike-RBD region Peroxidase conjugated antibody produced in rabbit

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) or (2019-nCoV) is a novel coronavirus that had spread on December 2019 in Hubei province of China and infected millions of people worldwide.1 The causative agent of COVID-19, the SARS-CoV-2 virus is a positive-strand RNA virus .The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S), E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The main receptor for SARS-CoV and SARS-CoV-2 on the membrane of the target cells is the Angiotensin 2 Converting Enzyme (ACE2). ACE2 is a metallopeptidase present on the membrane of many cells, including type-I and -II pneumocytes, small intestine enterocytes, kidney proximal tubules cells, the endothelial cells of arteries and veins, and the arterial smooth muscle, among other tissues.15-16 It has been shown that SARS-CoV-2 virus employs transmembrane protease serine 2 (TMPRSS2) for S protein priming and it is speculated that furin-mediated cleavage at the S1/S2 site in infected cells, may promote subsequent TMPRSS2-dependent entry into target cells.

Anti-SARS-CoV-2-Spike-RBD region antibody specifically recognizes Spike from COVID-19 virus origin.

The antibody may be used in various immunochemical techniques including Immunoblotting and Elisa. Detection of the Spike RBD protein band by Immunoblotting is specifically inhibited by the immunogen.

The Spike protein (S) is responsible for virus binding and entry into the host cells. SARS-CoV-2 S protein precursor is cleaved into S1 subunit (685 amino acids), and S2 (588 amino acids) subunits. S1 subunit harbor the receptor binding domain (RBD) that mediates virus entry into susceptible cells through the peptidase domain of host ACE2 with high affinity (Kd = 15 nM). S2 protein, which is reported to be well-conserved and showing 99% identity with bat coronavirus, is responsible for the membrane fusion. The Spike protein is the most studied between the coronaviruses proteins, due to its crucial role in the host cell entry, it contains the RBD for the ligand on the host cell membrane (the ACE2 protein), and also has epitopes recognized by T and B cells, which induce the production of neutralizing antibodies.2

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °CC for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

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