Anti-Enterococcus faecalis antibody produced in rabbit

Enterococcus faecalis is a gram positive, non-motile, facultative anaerobic, coci-shaped bacterium.1,2 It is a commensal bacterium of the human gut and a major opportunistic pathogen in susceptible hosts.1 E. faecalis is the third most common pathogen associated with hospital-acquired infections.2 E. faecalis inhabits the gut in 80% of healthy adultsand is also a part of the oral cavity normal flora in 20% of the adult population. In addition, E. faecalis was also identified in the human urinary tract.1

Anti-Enterococcus faecalis antibody recognizes E. faecalis lysate and whole dead bacteria

The antibody may be used in various immunochemical techniques including Immunoblotting. Detection of the E. faecalis bands by Immunoblotting is specifically inhibited by the immunogen.

E. faecalis contributes to the host in several ways, such as, competing with pathogen bacteria and preventing them from reaching the gut epithelia, prevention of intestinal epithelial barrier leakage and provision of protection from pathogens to the young infant through breast milk consumption.1,3-5
Moreover, E. faecalis also has many effects on the immune system that are beneficial to the host, such as, promoting the secretion of anti-inflammatory cytokines IL-10 and TGF-ß and induction of IgA production.1,6,7
However, when the homeostasis in the gut and mucus barrier is disrupted, especially in susceptible individuals such as in inflammatory bowel diseases (IBD) patients, or upon antibiotic administration, E. faecalis might become an opportunistic pathogen. In such case, the bacteria population overgrows and translocates through the mucus barrier to reach the epithelial cells of the gut where it can enter the bloodstream and the lymphatic system and cause severe inflammation.

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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