SAB4200856
Anti-Mitofilin antibody, Mouse monoclonal
clone BNP-28, purified from hybridoma cell culture
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UNSPSC Code:
12352203
NACRES:
NA.43
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antibody form
purified from hybridoma cell culture
Quality Level
clone
BNP-28, monoclonal
form
liquid
species reactivity
rat, mouse, bovine, human
concentration
~1 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using whole extracts of human liver cancer cell line HePG2
immunofluorescence: 5-10 μg/mL using human liver cancer cell line HePG2
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Mitochondria are essential organelles whose function is critical to homeostasis, energy generation, and cell fate.
Their dysfunction leads to various human diseases. Mitochondria membrane is composed of the outer membrane (OMM) and the inner membrane (IMM) where the electron transport chain machinery takes place and the cristae folds of the mitochondria are formed.1 Mitofilin, a transmembrane IMM protein, is a critical core component of the mitochondrial contact site and cristae organizing system (MICOS).Mitofilin is expressed preferentially in the heart3 but also in the brain4, liver5, kidneys6 and eyes7. It is abundantly found at mitochondrial cristae junctions and is also present within cristae.8,9
Their dysfunction leads to various human diseases. Mitochondria membrane is composed of the outer membrane (OMM) and the inner membrane (IMM) where the electron transport chain machinery takes place and the cristae folds of the mitochondria are formed.1 Mitofilin, a transmembrane IMM protein, is a critical core component of the mitochondrial contact site and cristae organizing system (MICOS).Mitofilin is expressed preferentially in the heart3 but also in the brain4, liver5, kidneys6 and eyes7. It is abundantly found at mitochondrial cristae junctions and is also present within cristae.8,9
Specificity
Monoclonal Anti-Mitofilin antibody specifically recognizes human and hamster Mitofilin.
Application
The antibody may be used in various immunochemical techniques including Immunoblotting (~85 kDa) and Immunofluorescence. Detection of the Mitofilin band by Immunoblotting is specifically inhibited by the immunogen.
Biochem/physiol Actions
Mitofilin has critical roles in cristae organization, MICOS formation, ATP generation, protein transport, mitochondrial DNA transcription and apoptosis.1
Mitofilin downregulation or misexpression has been associated with various human diseases such as, diabetes, Parkinson′s and Alzheimer′s diseases, heart failure, hepatic carcinoma, gastric cancer and Glaucoma.1
Mitofilin downregulation or misexpression has been associated with various human diseases such as, diabetes, Parkinson′s and Alzheimer′s diseases, heart failure, hepatic carcinoma, gastric cancer and Glaucoma.1
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Xiaorong Yang et al.
Biophysics reports, 4(2), 104-113 (2018-05-15)
There is accumulating evidence that cysteine sulfenation (cys-SOH) in proteins plays an important role in cellular response to oxidative stress. The purpose of the present study was to identify mitochondrial proteins that undergo changes in cys-SOH during aging. Studies were
Yurong Guo et al.
Molecular & cellular proteomics : MCP, 12(12), 3744-3758 (2013-09-14)
Insulin resistance plays a major role in the development of type 2 diabetes and obesity and affects a number of biological processes such as mitochondrial biogenesis. Though mitochondrial dysfunction has been linked to the development of insulin resistance and pathogenesis
Daniel C Jans et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(22), 8936-8941 (2013-05-17)
The mitochondrial inner membrane organizing system (MINOS) is a conserved large hetero-oligomeric protein complex in the mitochondrial inner membrane, crucial for the maintenance of cristae morphology. MINOS has been suggested to represent the core of an extended protein network that
Yansheng Feng et al.
Journal of cellular physiology, 234(4), 3383-3393 (2018-09-28)
The identification of the mitochondrial contact site and cristae organizing system (MICOS) in the inner mitochondrial membrane shed light on the intricate components necessary for mitochondria to form their signature cristae in which many protein complexes including the electron transport
Curtis L Nordgaard et al.
Investigative ophthalmology & visual science, 49(7), 2848-2855 (2008-03-18)
Age-related macular degeneration (AMD) is the leading cause of vision loss in individuals over the age of 65. Histopathological changes become evident in the retinal pigment epithelium (RPE), a monolayer that provides metabolic support for the overlying photoreceptors, even at
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