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SAB4200817

Sigma-Aldrich

Anti- Proteus vulgaris antibody produced in rabbit

IgG fraction of antiserum

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

antibody form

IgG fraction of antiserum

clone

polyclonal

description

Research area: Microbiome

form

buffered aqueous solution

mol wt

~70 kDa

species reactivity

Proteus vulgaris

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 1:20,000-1:40,000 using Proteus vulgaris LPS.
indirect ELISA: 1:20,000-1:40,000 using dead Proteus vulgaris bacteria for coating.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Proteus vulgaris is a Gram negative rod-shaped bacteria, belongs to the Enterobacteriaceae family. Members of the genus Proteus (Proteus spp.) which also includes Proteus mirabilis, Proteus penneri and Proteus hauseri, originally characterize by their ability to swarm on solid surfaces, are widespread in the environment and the gastrointestinal tract of human and animals and known to be an opportunistic pathogens isolated from urine, wounds and other clinical sources. The Proteus spp. bacteria, are distinguished by their reactions for indole production, salicin fermentation and aesculin hydrolysis. P. vulgaris produces indole which differentiates it from the indole-negative P. mirabilis and P. penneri. Proteus spp. bacteria may also be found in soil or water habitats where they often regarded as indicators of fecal pollution and a contamination threat for potential water or seafood poisoning.

Immunogen

Proteus vulgaris dead OX19 bacteria: ATCC strain 6380

Application

Anti-Proteus vulgaris antibody recognizes P. vulgaris whole extract and P. vulgaris LPS, the antibody also recognizes an additional ~70kDa band suspected as bacterial HSP70 (DNAK) in whole extract P. mirabilis, P. gingivalis, E.coli K-12, P.aeruginosa, S. flexneri, S. enterica and E. faecalis but it has no cross reactivity with P. mirabilis LPS. The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA. 

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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The effect of p-nitrophenylglycerol on swarming and the production of some virulence factors in Proteus vulgaris
Ghaidaa M, et al.
New York Science Journal, 6, 8-14 (2013)
C M O'Hara et al.
International journal of systematic and evolutionary microbiology, 50 Pt 5, 1869-1875 (2000-10-18)
Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as Proteus penneri. Biogroup 2, characterized by positive reactions for indole, salicin and aesculin
ANTIMICROBIAL RESISTANCE PATTERNS OF PROTEUS ISOLATES FROM CLINICAL SPECIMENS
Bahashwan, et al.
EUROPEAN SCIENTIFIC JOURNAL, 9, 188-202 (2013)
N Pal et al.
Annals of medical and health sciences research, 6(5), 267-273 (2017-05-16)
Proteus species cause a variety of community- and hospital-acquired illnesses. Synthesis of β-lactamases is the predominant mechanism for resistance to β-lactam antibiotics. Among the β-lactamases, extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases are the most common. The objective of this
C M O'Hara et al.
Clinical microbiology reviews, 13(4), 534-546 (2000-10-12)
This review presents the current taxonomy of the genera Proteus, Providencia, and Morganella, along with the current methods for the identification of each species within the three genera, incorporating both conventional biochemical and commercial methods. While all of these organisms

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