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SAB4200809

Sigma-Aldrich

Anti-Epidermal Growth Factor (EGF) Receptor, Mouse monoclonal

clone 29.1, purified from hybridoma cell culture

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Synonym(s):
EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

29.1, monoclonal

form

buffered aqueous solution

mol wt

~170 kDa

species reactivity

human, bovine, mouse

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL using human epidermoid carcinoma A431 cell line extract.
immunofluorescence: 2-4 μg/mL using human epidermoid carcinoma A431 cells.

isotype

IgG1

UniProt accession no.

P00533

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

General description

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity. EGF binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue.

Immunogen

Paraformaldehyde-fixed carcinoma cell line A431

Application

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor specifically bind to a carbohydrate residue on the external region of the EGF receptor molecule with no interference to the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to the EGF Receptor. Reactivity has been observed with EGF receptors from mouse, human and bovine origin. The antibody is recommended to use in various immunological techniques, including Immunobloting (~170 kDa), Immunoprecipitation, flow cytometry (FACS), Immunohistochemistry and Immunofluorescence.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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