SAB4200798
Anti-GSDMD antibody, Mouse monoclonal
clone GSD-14, purified from hybridoma cell culture
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Synonym(s):
Anti-Gasdermin domain-containing protein 1, Anti-Gasdermin-D
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
GSD-14, monoclonal
form
buffered aqueous solution
mol wt
~53 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using human Jurkat cell extract
immunofluorescence: 5-10 μg/mL using human A431 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GSDMD(79792)
General description
GSDMD (gasdermin D) also known as DFNA5L or Gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in the upper gastrointestinal epithelium differentiating cells and has been suggested to act as a tumor suppressor since it is suppressed in high percentage of esophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gramnegative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1β processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that Caspase-4/11 specifically cleaves GSDMD after Asp275, leading to gasdermin-N domain that bears intrinsic pyroptosisinducing activity. 4 Furthermore, GSDMD-mediated pyroptosis plays an important role in mature IL-1β release without affecting its maturation.
Monoclonal Anti-GSDMD recognizes human GSDMD.
Monoclonal Anti-GSDMD recognizes human GSDMD.
Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.
Immunogen
Synthetic peptide of human GSDMD, conjugated to KLH
Application
The antibody is recommended to use in various immunological techniques, including Immunoblot (∼53 kDa) and Immunofluorescence.
Biochem/physiol Actions
Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
WGK
WGK 3
Regulatory Information
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Inflammatory caspase-related pyroptosis: mechanism, regulation and therapeutic potential for inflammatory bowel disease
Kayagaki N, et al.
Gastroenterology, 526(7575), 666-666 (2015)
Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling
Kayagaki N, et al.
Nature, 526(7575), 666-666 (2015)
Wan-ting He et al.
Cell research, 25(12), 1285-1298 (2015-11-28)
Inflammasome is an intracellular signaling complex of the innate immune system. Activation of inflammasomes promotes the secretion of interleukin 1β (IL-1β) and IL-18 and triggers pyroptosis. Caspase-1 and -11 (or -4/5 in human) in the canonical and non-canonical inflammasome pathways
Caina Ma et al.
Virologica Sinica, 37(3), 445-454 (2022-05-06)
African swine fever (ASF) is a highly pathogenic swine infectious disease that affects domestic pigs and wild boar, which is caused by the African swine fever virus (ASFV). ASF has caused huge economic losses to the pig industry and seriously
Members of a novel gene family, Gsdm, are expressed exclusively in the epithelium of the skin and gastrointestinal tract in a highly tissue-specific manner
Tamura M, et al.
Genomics, 89(5), 618-629 (2007)
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