SAB4200782
Anti-Tenascin antibody, Mouse monoclonal
clone BC-24, purified from hybridoma cell culture
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Synonym(s):
Anti-Cytotactin, Anti-GMEM, Anti-GP 150-225, Anti-Glioma-associated-extracellular matrix antigen, Anti-Hexabrachion, Anti-JI, Anti-Myotendinous antigen, Anti-Neuronectin, Anti-TN, Anti-Tenascin-C (TN-C)
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
BC-24, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
ELISA: suitable
flow cytometry: suitable
immunoblotting: suitable
immunohistochemistry: 5-10 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tonsil sections
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TNC(3371)
Related Categories
General description
Monoclonal Anti-Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human tenascin. Tenascin-C (TN-C) is also known as Hexabrachion, Cytotactin, Neuronectin (NEC1). It is a high molecular mass extracellular matrix glycoprotein. Human tenascin is a disulfide-linked hexamer composed of 3 subunits of 190, 200, and 220 kDa.
Immunogen
Human tenascin
Application
Anti-Tenascin antibody has been used:
- in immunoblotting
- in Immunohistochemistry
- in flow cytometry
- in enzyme linked immunosorbent assay(ELISA)
- for blocking tenascin-C proliferating activity
Biochem/physiol Actions
Tenascin-C (TN-C) functions in cell adhesion, fibroblast migration, and other processes related to tissue remodeling and wound healing. It has been proposed that actively growing, migrating and differentiating epithelial sheets can produce factors such as (Transforming growth factor beta) TGF-β to stimulate tenascin expression. Neo-expression or increased expression of tenascin has been found in the stroma of various tumors and during normal tissue repair. Intracytoplasmic tenascin immunoreactivity has been detected in malignant melanomas and in lung carcinomas, and it serves as a marker of stromal element proliferation in invasive breast carcinomas. High-molecular mass tenascin isoform plays a role in generating a permissive environment for proliferation, invasion, and metastasis of neoplastic epithelial cells. Human tenascin contains an Arg-Gly-Asp (RGD) sequence which may function in cell adhesion and mediates cell attachment through an RGD-dependent integrin receptor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Tenascin: cDNA cloning and induction by TGF-beta.
Pearson C A, et al.
The Embo Journal, 7(10), 2977-2982 (1988)
Christopher R Silvers et al.
British journal of cancer, 125(10), 1399-1407 (2021-09-27)
Markers of stromal activation at future metastatic sites may have prognostic value and may allow clinicians to identify and abolish the pre-metastatic niche to prevent metastasis. In this study, we evaluate tenascin-C as a marker of pre-metastatic niche formation in
The role of tenascin-C in tissue injury and tumorigenesis
Midwood K S, et al.
Journal of Cell Communication and Signaling, 3(3-4), 287-310 (2009)
Tenascin-C modulates tumor stroma and monocyte/macrophage recruitment but not tumor growth or metastasis in a mouse strain with spontaneous mammary cancer
Talts J F, et al.
Journal of Cell Science, 112(12), 1855-1864 (1999)
Rintu Thomas et al.
Cell communication and signaling : CCS, 20(1), 119-119 (2022-08-11)
Bone metastatic prostate cancer does not completely respond to androgen-targeted therapy and generally evolves into lethal castration resistant prostate cancer (CRPC). Expression of AR-V7- a constitutively active, ligand independent splice variant of AR is one of the critical resistant mechanisms
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