SAB4200755
Anti-Human IgE-Peroxidase antibody, Mouse monoclonal
clone GE-1, purified from hybridoma cell culture
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Synonym(s):
Anti-Immunoglubulin E
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
GE-1, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
ELISA: 1:20,000-1:30,000 using 5 μg/mL Human IgE myeloma for coating
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgE myeloma protein. IgE (Immunoglobulin E) is the least abundant immunoglobulin isotype. IgE is produced by plasma cells and is highly functional in the lung, skin, and mucous membranes. Normal levels of IgE in serum are less than 1 μmL.
Specificity
Monoclonal Anti-Human IgE specifically recognizes Human IgE. The antibody shows no cross-reactivity with human IgG1, IgG2, IgG3, IgG4, IgA, and IgM.
Immunogen
Purified human IgE
Application
Anti-Human IgE-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
IgE plays a critical role in allergic reactions and host protection from parasites. During an allergic reaction such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease, or primary immunodeficiency the serum IgE levels can be induced up to 5-fold. IgE specifically and reversibly binds to the membrane receptor Fc-epsilon receptors (FcεR) type I on the surface of basophils, eosinophils, and mast cells. This binding activates the release of histamine, leukotrienes, and other mediators responsible for hypersensitivity. The recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.
Physical form
Supplied as a lyophilized powder.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
IgE and mast cells in allergic disease
Wachholz P A, et al.
Journal of Immunotoxicology, 1(3-4), 189-199 (2005)
IgE and mast cells in allergic disease
Galli S J and Tsai M
Nature Medicine, 18(5), 693-693 (2012)
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