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SAB4200747

Sigma-Aldrich

Anti-Neurofilament 200 antibody, Mouse monoclonal

clone NE14, purified from hybridoma cell culture

Synonym(s):

Anti-H-subunit, Anti-NF-H

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NE14, monoclonal

form

buffered aqueous solution

mol wt

~200 kDa

species reactivity

feline, rat, guinea pig, pig, human, mouse, bovine, chicken

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction
immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections

isotype

IgG1

UniProt accession no.

P12036

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Nefh(528842)
cat ... Nefh(101087272)
chicken ... Nefh(417020)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

Related Categories

General description

Anti-Neurofilament 200 Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NE14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as neurofilament heavy polypeptide (Hsubunit), NF-H, NEFH or 200 kDa neurofilament protein.

Immunogen

Neurofilaments purified from pig spinal cord

Application

Anti-Neurofilament 200 antibody, Mouse monoclonal has been used in immunoblotting and immunohistochemistry staining.

Biochem/physiol Actions

Neurofilaments are the type of intermediate filaments (IFs), that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. It has an important function in mature axons that is not sub served by the two smaller neurofilament proteins. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion. Neurofilaments can accumulate in large numbers within cell bodies and proximal axons of affected neurons in several pathological diseases, such as Charcot-Marie-Tooth (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, neurofilament accumulations was detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Xinghui Wang et al.
Molecular medicine reports, 19(5), 4377-4387 (2019-04-04)
Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) is a molecular biological method used to assess gene expression characterized by high simplicity, effectiveness, specificity and sensitivity. The selection of a suitable reference gene for normalization is critical for the accuracy of quantitative
Plasma neurofilament heavy chain levels correlate to markers of late stage disease progression and treatment response in SOD1G93A mice that model ALS
Lu CH, et al.
Testing, 7(7), e40998-e40998 (2012)
Identification of suitable reference genes for gene expression studies in rat skeletal muscle following sciatic nerve crush injury
Wang X, et al.
Molecular Medicine Reports, 19(5), 4377-4387 (2019)
Ben G Szaro et al.
Trends in neurosciences, 33(1), 27-37 (2009-11-13)
Neurofilament (NF) protein expression is coupled to axon development and the maintenance of neuronal homeostasis. Here, we present evidence that this tight regulation depends critically on post-transcriptionally regulated changes in NF mRNA transport, translation and stability. Recent studies have shown

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