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SAB4200740

Sigma-Aldrich

Anti-Neurofilament 160 antibody, Mouse monoclonal

clone NN18, purified from hybridoma cell culture

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Synonym(s):
Anti-160 kDa neurofilament protein, Anti-NF-M, Anti-Neurofilament 3, Anti-Neurofilament triplet M protein
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NN18, monoclonal

form

buffered aqueous solution

mol wt

antigen ~160 kDa

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.3-0.6 μg/mL using rat brain extract
immunofluorescence: 10-20 μg/mL using human bone marrow neuroblast SHSY5Y cell line
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain sections

isotype

IgG1

UniProt accession no.

P07197

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Anti-Neurofilament 160 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the NN18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with neurofilaments purified from pig spinal cord. Neurofilaments (NFs) belong to the intermediate filament (IF) family and are expressed mainly in cells or tissues of neuronal origin. Three major NFs subunits were discovered: light (NF-L, ~68 kDa), medium (NF-M, ~160 kDa) and heavy (NF-H, ~200 kDa), which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 160, also known as NF-M or Neurofilament triplet M protein.

Immunogen

neurofilaments purified from pig spinal cord

Application

Anti-Neurofilament 160 antibody has been used in immunoblotting, Immunohistochemistry, Immunofluorescence

Biochem/physiol Actions

NFs can accumulate in large numbers within cell bodies and proximal axons of affected neurons in a variety of pathologies, including Charcot-Marie-Tooth disease (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, NFs accumulation were detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. Elevated concentrations of Neurofilament 160 have been shown in cerebrospinal fluid (CSF) and serum samples from patients with brain injury.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Elevated neurofilament levels in neurological diseases
Norgren N, et al.
Brain Research, 987(1), 25-31 (2003)
Neurofilaments and neurological disease
Al-Chalabi A and Miller C C
Bioessays, 25(4), 346-355 (2003)

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