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SAB4200728

Sigma-Aldrich

Anti-Chromogranin-A antibody, Mouse monoclonal

clone CHGA(419), purified from hybridoma cell culture

Synonym(s):

Anti-CHGA, Anti-CgA, Anti-Pituitary secretory protein I, Anti-SP-1

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CHGA(419), monoclonal

form

buffered aqueous solution

mol wt

~50 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 10-20 μg/mL using human embryonic kidney 293T cell extract
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded Human Stomach sections

isotype

IgG1

UniProt accession no.

P10645

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHGA(1113)

General description

Anti-Chromogranin-A antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CHGA(419) hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide corresponding to the C-terminal region of human Chromogranin-A protein, conjugated to KLH. Chromogranin-A protein, also known as CgA, pituitary secretory protein I (SP-1) is the major member of the granin family of acidic secretory glycoproteins that are expressed in all endocrine and neuroendocrine cells. The protein is in neuroendocrine cells distributed throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets and the secretory vesicles of neurons.

Immunogen

synthetic peptide corresponding to the C-terminal region of human Chromogranin-A protein conjugated to KLH

Application

Anti-Chromogranin-A antibody has been used in immunohistochemistry,immunoblotting.

Biochem/physiol Actions

Chromogranin-A has a crucial intracellular role in secretory granule biogenesis and calcium homeostasis. Tissue-specific and context-specific proteolytic cleavage of Chromogranin-A yields polypeptides with paracrine and endocrine activity including: vasostatin, catestatin, GE25 and WE-14. Increased blood levels of Chromogranin-A have been shown in numerous inflammatory and non-inflammatory conditions, including neuroendocrine tumours (carcinoid tumors, phenochromocytomas, paragangliomas and others), renal failure, arterial hypertension, chronic heart failure and rheumatoid arthritis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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Chromogranin A: its clinical value as marker of neuroendocrine tumours.
Nobels FR, et al.
European Journal of Clinical Investigation, 28(6), 431-440 (1998)
Chromogranin-A production and fragmentation in patients with Takayasu arteritis
Tombetti E, et al.
Arthritis Research & Therapy, 18(1), 187-187 (2016)
The endocrine role for chromogranin A: a prohormone for peptides with regulatory properties
Helle KB, et al.
Cellular and Molecular Life Sciences, 64(22), 2863-2886 (2007)
A new human chromogranin A (CgA) immunoradiometric assay involving monoclonal antibodies raised against the unprocessed central domain (145-245)
Degorce F, et al.
British Journal of Cancer, 79(1), 65-65 (1999)
Gervaise H Henry et al.
Cell reports, 25(12), 3530-3542 (2018-12-20)
A comprehensive cellular anatomy of normal human prostate is essential for solving the cellular origins of benign prostatic hyperplasia and prostate cancer. The tools used to analyze the contribution of individual cell types are not robust. We provide a cellular

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