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Merck
CN

SAB4200719

Anti-Anti-Laminin (LAMA1) antibody

mouse monoclonal, LAM-89

Synonym(s):

Anti-LAMA, Anti-LAMA1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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Product Name

Anti-Laminin antibody, Mouse monoclonal, clone LAM-89, purified from hybridoma cell culture

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

LAM-89, monoclonal

form

buffered aqueous solution

mol wt

~850 kDa

species reactivity

feline, human, porcine

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
electron microscopy: suitable
immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
immunofluorescence: suitable
immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.

isotype

IgG1

UniProt accession no.

P25391

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

cat ... Lama1(101083299)
human ... LAMA1(284217)
pig ... Lama1(100625589)

Related Categories

Application

Monoclonal Anti-Laminin is recommended to use in various immunochemical assays, including immunohistochemistry, immunoblotting (~850 kDa), immunofluorescence, Enzyme linked immunosorbent assay (ELISA) and electron microscopy.

Biochem/physiol Actions

Laminins contribute to the structure of the extracellular matrix (ECM). It also plays a major role in the regulation of variety of cellular processes, such as the rates of cell proliferation, differentiation, adhesion, migration, cell shaping, phenotype stability and resistance to apoptosis. Laminin polymers forms supramolecular network with collagen IV polymers through heparan sulfate proteoglycan and by nidogen (entactin) and plays an important role in the basement membrane (BM) stability. In mouse, mutations in the gene is associated with the development of muscular dystrophy.
Monoclonal Anti-Laminin antibody specifically recognizes Laminin from human, feline and porcine origin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta. Monoclonal anti-laminin antibody specifically recognizes laminin from human, feline and porcine origin. The antibody shows reactivity with laminin and specifically staining basal membrane of blood vessels, epithelium, nerve and muscle fiber. Monoclonal anti-laminin shows no reaction with collagen IV, fibronectin, vitronectin and chondroitin sulfate type A, B or C. Laminins are complexed extracellular glycoproteins which assemble the basal components of the basement membrane (BM). Each laminin is a heterotrimer (~850kDa) comprising of an α (~400kDa), β (~200kDa) and γ (~200kDa) chains all held together by disulfide bonds.

Immunogen

purified Laminin from human Placenta

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000417631
0000417631
0000252631
0000222581
205051

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Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) gene.
Xu H, et al.
Nature Genetics, 8, 297-302 (1994)
Basic components of connective tissues and extracellular matrix: elastin, fibrillin, fibulins, fibrinogen, fibronectin, laminin, tenascins and thrombospondins.
Halper J and Kjaer M
Advances in Experimental Medicine and Biology, 31-47 (2014)
Laminins: Roles and Utility in Wound Repair.
Iorio V, et al.
Advances in Wound Care, 4, 250-263 (2015)
The laminin family
Aumailley M, et al.
Cell adhesion & migration, 7, 48-55 (2013)
Junya Takegaki et al.
Scientific reports, 11(1), 21224-21224 (2021-10-29)
Skeletal muscle mass is critical for good quality of life. Mesenchymal stem cells (MSCs) are multipotent stem cells distributed across various tissues. They are characterized by the capacity to secrete growth factors and differentiate into skeletal muscle cells. These capabilities
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