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SAB4200707

Sigma-Aldrich

Anti-Desmin antibody, Mouse monoclonal

enhanced validation

clone DE-U-10, purified from hybridoma cell culture

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Synonym(s):
Anti-DES
UNSPSC Code:
12352203
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

Quality Level

200

antibody product type

primary antibodies

clone

DE-U-10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, rabbit, hamster, porcine, goat, bovine, chicken, toad, sheep, mouse, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.5-1 μg/mL using whole extract of mouse myoblast C2C12 cells
immunofluorescence: 2-5 μg/mL using mouse myoblast C2C12 cells
immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded Human Appendix
microarray: suitable

isotype

IgG1

UniProt accession no.

P02540

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DES(1674)
mouse ... Des(13346)
rat ... Des(64362)

Related Categories

General description

Anti-Desmin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma DE-U-10 produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Desmin, a major intermediate filament protein in skeletal, cardiac and smooth muscle cells. It is predominantly localised to filamentous extrasarcomeric cytoskeleton, which links neighbouring myofibrils and connects the myofibrillar apparatus with the subsarcolemmal cytoskeleton, myonuclei and mitochondria.

Immunogen

Desmin purified from pig stomach

Application

Anti-Desmin antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • microarray

Biochem/physiol Actions

Mutations is the desmin gene (known as desminopathy or DRM) are associated with several human diseases such as atrophy and facial muscle weakness, cardiomyopathy, cardiac arrhythmias and restrictive heart failure and are characterized by intracellular desmin aggregates. Anti-Desmin antibodies can be implemented for cancer research and diagnostics since elevated levels of desmin characterize several types of muscle tissue tumors, such as leiomyomas and rhabdomyosarcomas. The antibody may also be used for meat research measurements.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chuan Xu et al.
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Interferon ɛ (IFNɛ) is a unique type I IFN that has been implicated in host defense against sexually transmitted infections. Zika virus (ZIKV), an emerging pathogen, can infect the female reproductive tract (FRT) and cause devastating diseases, particularly in pregnant
Yuan H Brad Kim et al.
Meat science, 95(2), 412-418 (2013-06-12)
Forty eight lamb carcasses with temperature and pH monitored were obtained from two commercial plants. At 24h post mortem both loins (M. longissimus) from each carcass were randomly allocated to a) unaged frozen at -18°C, (b) aged at -1.5°C for
Harald Bär et al.
Journal of structural biology, 148(2), 137-152 (2004-10-13)
Desmin, the major intermediate filament (IF) protein of muscle, is evolutionarily highly conserved from shark to man. Recently, an increasing number of mutations of the desmin gene has been described to be associated with human diseases such as certain skeletal
The physiological role of cardiac cytoskeleton and its alterations in heart failure
Sequeira V, et al.
Biochimica et Biophysica Acta - Biomembranes, 1838(2), 700-722 (2014)
Tumor markers: An overview
Babu GS, et al.
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, 4(2), 87-87 (2012)
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