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SAB4200673

Sigma-Aldrich

Monoclonal Anti-α-Amylase 1A antibody produced in mouse

clone AMY-7, purified from hybridoma cell culture

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Synonym(s):
AMY1, AMY1A, alpha 1A (salivary), amylase
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AMY-7, monoclonal

form

buffered aqueous solution

mol wt

~58 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using rat pancreas tissue extracts.
immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AMY1A(276)
mouse ... Amy1(11722)
rat ... Amy1a(24203)

Related Categories

General description

Monoclonal Anti- α-Amylase 1A (mouse IgG1 isotype) is derived from the hybridoma AMY-7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an N-terminal His-tagged recombinant human α. α-Amylase 1A, also known as AMY1A belongs to the α-Amylase family. AMY1A is a secreted digestive enzyme produced by the salivary glands and pancreas.

Immunogen

N-terminal His-tagged recombinant human α-Amylase 1A.

Application

Monoclonal Anti-α-Amylase 1A antibody has been used in immunoblotting, immunohistochemistry.

Biochem/physiol Actions

α-Amylase 1A cleaves the glycosidic linkages in starch molecules to produce smaller saccharides such as maltotriose, maltose, and small amounts of glucose. AMY1A has been identified as an indicator in pancreas and salivary gland diseases, as well as lung cancer, ovarian cancer, plasmacytoma and thyroid adenomas.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Hyposalivation-related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid
Subsite mapping of human salivary alpha-amylase and the mutant Y151M
Kandra L, et al.
Febs Letters, 544(1-3), 194-198 (2003)
Expression of alpha-amylase isozymes in human thyroid tissues
Doi S, et al.
Cancer research, 51(13), 3544-3549 (1991)
Xian Zhang et al.
Developmental cell, 57(12), 1496-1511 (2022-06-09)
Diabetic patients show elevated plasma IL18 concentrations. IL18 has two receptors: the IL18 receptor (IL18r) and the Na-Cl co-transporter (NCC). Here, we report that IL18 is expressed on islet α cells, NCC on β cells, and IL18r on acinar cells

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