SAB4200669
Anti-Digoxin antibody, Mouse monoclonal
clone DI-22, purified from hybridoma cell culture
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12β-Hydroxydigitoxin, Monoclonal Anti-Digoxin antibody produced in mouse
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biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DI-22, monoclonal
species reactivity
human, mouse, rat
concentration
1.0 mg/mL
~1 mg/mL
technique(s)
ELISA: 0.15-0.3 μg/mL
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
General description
Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with digoxin conjugated to keyhole limpet hemocyanin (KLH).
Immunogen
immunized with Digoxin
Application
Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- flow cytometry
- immunofluorescence
- DNA hybridization
- in-situ hybridization (ISH)
Biochem/physiol Actions
Digoxin is an uncharged glycoside which is given to humans with congestive heart failure. It enhances the force of myocardial contraction. Anti-digoxin antibodies can be used to reverse the effects of digoxin.
Unlike other oral inotrope, digoxin does not promote mortality. It inhibits sodium potassium ATPase membrane pump.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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High-affinity monoclonal antibodies to the cardiac glycoside, digoxin.
Journal of immunology (Baltimore, Md. : 1950), 129(3), 1165-1172 (1982-09-01)
Digoxin
Progress in Cardiovascular Diseases, 44(4), 251-266 (2002)
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