SAB4200657
Monoclonal Anti-EEF1A1 antibody produced in mouse
clone EF48, purified from hybridoma cell culture
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Synonym(s):
CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, HNGC:16303, LENG7, PTI1, eEF1A-1, eukaryotic translation elongation factor 1 α 1
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
EF48
form
buffered aqueous solution
species reactivity
bovine, mouse, rat, human, monkey, canine
concentration
~1 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells.
immunofluorescence: 0.5-1 μg/mL using SW-620 cells.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EEF1A1(1915)
mouse ... Eef1a1(13627)
rat ... Eef1a1(171361)
Related Categories
General description
Eukaryotic translation elongation factor 1 α 1 (EEF1A1) is a cellular protein which is part of the eukaryotic elongation factor 1 (eEF1) complex. The gene encoding it is localized on human chromosome 6q14.1.
Monoclonal Anti-EEF1A1 (mouse IgG2b isotype) is derived from the hybridoma EF48 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Eukaryotic elongation factor 1 A-1 (EEF1A1), also known as EF-l α is a GTP-binding protein. EEF1A1 is one of the most abundant cytoplasmic proteins and is ubiquitously expressed except in neurons and muscle.
Immunogen
a synthetic peptide corresponding to an internal sequence of human EEF1A1
Application
Monoclonal Anti-EEF1A1 antibody produced in mouse has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Eukaryotic elongation factor 1 A-1 (EEF1A1) is involved in proper codon-anticodon binding interactions between mRNAs and tRNAs at the tRNA acceptor site of the ribosome. EEF1A also plays a translation-independent role in embryogenesis, senescence, oncogenic transformation, cell proliferation, apoptosis, cytoskeletal organization and protein degradation. Overexpression of EEF1A1 has been observed in various tumors of the pancreas, colon, breast, lung, and stomach.
Eukaryotic translation elongation factor 1 α 1 (EEF1A1) binds to aminoacetylated tRNAs and delivers them to the elongating ribosome during protein synthesis. It aids in the association of the eukaryotic elongation factor 1 (eEF1) complex with the HIV-1 (human immunodeficiency virus-1) reverse transcription complex. EEF1A1 also associates with genomic RNA secondary structures of RNA viruses and helps in their replication.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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The ubiquitin-like protein FAT10 stabilizes eEF1A1 expression to promote tumor proliferation in a complex manner
Liu X, et al.
Cancer research, 76(16), 4897-4907 (2016)
A Lamberti et al.
Amino acids, 26(4), 443-448 (2004-08-04)
An increasing number of evidences suggest the involvement of the eukaryotic elongation factor 1A, a core component of the protein synthesis machinery, at the onset of cell transformation. In fact, eEF1A is shown to be up-regulated in cell death; moreover
Highly homologous eEF1A1 and eEF1A2 exhibit differential post-translational modification with significant enrichment around localized sites of sequence variation
Soares DC and Abbott CM
Biology Direct, 8(1), 29-29 (2013)
Molecular characterization and expression patterns of porcine eukaryotic elongation factor 1 A
H.L. Wang
Asian-Australasian Journal of Animal Sciences, 19(7), 953-953 (2006)
Dongsheng Li et al.
PLoS pathogens, 11(12), e1005289-e1005289 (2015-12-02)
Reverse transcription is the central defining feature of HIV-1 replication. We previously reported that the cellular eukaryotic elongation factor 1 (eEF1) complex associates with the HIV-1 reverse transcription complex (RTC) and the association is important for late steps of reverse
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