SAB4200641
Monoclonal Anti-Brg1 antibody produced in rat
clone 3G4, purified from hybridoma cell culture
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Synonym(s):
Anti-ATP-dependent helicase SMARCA4, Anti-BAF190, Anti-BAF190A, Anti-BRG1, Anti-BRG1-associated factor 190A, Anti-BRM/SWI2-related gene 1, Anti-MRD16, Anti-RTPS2, Anti-SMARCA4, Anti-SNF2, Anti-SNF2-beta, Anti-SNF2-like 4, Anti-SNF2L4, Anti-SNF2LB, Anti-SWI/SNF related, Anti-SWI2, Anti-actin dependent regulator of chromatin, Anti-brahma protein-like 1, Anti-global transcription activator homologous sequence, Anti-hSNF2b, Anti-homeotic gene regulator, Anti-matrix associated, Anti-member 4, Anti-mitotic growth and transcription activator, Anti-nuclear protein GRB1, Anti-protein BRG-1, Anti-protein brahma homolog 1, Anti-subfamily a, Anti-sucrose nonfermenting-like 4, Anti-transcription activator BRG1
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3G4, monoclonal
form
buffered aqueous solution
mol wt
~185 kDa
species reactivity
mouse, human
concentration
~1 mg/mL
technique(s)
flow cytometry: 2.5-5 μg/test using HeLa cells
immunoblotting: 0.1-0.2.0 μg/mL using HeLa nuclear extract
immunofluorescence: 10 μg/mL using HeLa cells
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BRG1(6597)
mouse ... Brg1(20586)
Related Categories
General description
Monoclonal Anti-Brg1 (rat IgG1 isotype) is derived from the hybridoma 3G4 produced by the fusion of mouse myeloma cells (SP2) and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human Brm-related gene-1 (Brg1) protein. Brg1 or SWI/SNF (SWItch/Sucrose non-fermentable) related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A, member 4 (SMARCA4) gene with 35 exons, is encoded by the gene mapped to human chromosome 19p13.
Immunogen
a synthetic peptide corresponding to a sequence at the C-terminal region of human Brg1 protein
Application
Monoclonal Anti-Brg1 antibody produced in rat has been used in:
- immunoblotting (~185 kDa)
- flow cytometry
- immunofluorescence
Biochem/physiol Actions
Brm-related gene-1 (Brg1) (or hBrm) protein is the central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, which is involved in transcriptional modulation of hormone-responsive promoters through binding of the complex to various nuclear receptors and its recruitment to gene-specific promoters. In addition, BRG1 has also been shown to act as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. BRG1 participates in the firing of origins and the process of replication elongation. This function of BRG1 also facilitates embryogenesis. In addition, It functions as a tumor suppressor and maintains genome stability and prevent cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
The BRG1 transcriptional coregulator
Trotter KW and Archer TK
Nuclear Receptor Signaling, 6(1), nrs-06004 (2008)
Mutation analysis of the BRG1 gene in prostate cancer clinical samples
Valdman A, et al.
International Journal of Oncology, 22(5), 1003-1007 (2003)
Stephanie M Cohen et al.
Nucleic acids research, 38(20), 6906-6919 (2010-06-24)
For DNA replication to occur, chromatin must be remodeled. Yet, we know very little about which proteins alter nucleosome occupancy at origins and replication forks and for what aspects of replication they are required. Here, we demonstrate that the BRG1
H S Zhang et al.
Cell, 101(1), 79-89 (2000-04-25)
We present evidence that Rb forms a repressor containing histone deacetylase (HDAC) and the hSWI/SNF nucleosome remodeling complex, which inhibits transcription of genes for cyclins E and A and arrests cells in the G1 phase of the cell cycle. Phosphorylation
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