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SAB4200617

Sigma-Aldrich

Monoclonal Anti-Myb antibody from mouse

clone MB-8, purified immunoglobulin

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Synonym(s):
Proto-oncogene c-Myb, Transcriptional activator Myb, Transcriptional activator Myb; Proto-oncogene c-Myb
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MB-8, monoclonal

species reactivity

human, mouse

concentration

~1 mg/mL

technique(s)

flow cytometry: 2.5-5 μg/test using using HL-60 cells.
immunoblotting: 0.5-1 μg/mL using using Jurkat total cell extract.
immunofluorescence: 2-4 μg/mL using using HeLa cells.

isotype

IgG1

UniProt accession no.

P10242

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYB(4602)

Related Categories

General description

Monoclonal Anti-Myb (mouse IgG1 isotype) is derived from the hybridoma MB-8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. MYB proto-oncogene, transcription factor is encoded by the gene mapped to human chromosome 6q23.3.

Specificity

Monoclonal Anti-Myb recognizes human and mouse Myb.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human Myb.

Application

Monoclonal Anti-Myb antibody from mouse may be used in:
  • immunoblotting
  • flow cytometry
  • immunocytochemistry

Biochem/physiol Actions

MYB is a transcription factor that regulates stem and progenitor cells in the bone marrow, colonic crypts and a neurogenic region of the adult brain. Loss of MYB activity in these cells leads to severe or lethal phenotypes. MYB is an oncogene and is observed in some human leukemias. In addition, It is also observed that MYB is activated in colon and breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 ˚C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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The HBS1L-MYB intergenic region on chromosome 6q23. 3 influences erythrocyte, platelet, and monocyte counts in humans
Menzel S, et al.
Blood, 110(10), 3624-3626 (2007)
MYB function in normal and cancer cells
Ramsay RG, et al.
Nature Reviews. Cancer, 8, 523-534 (2008)

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