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SAB4200600

Sigma-Aldrich

Monoclonal Anti-KHSRP antibody produced in mouse

clone KS-8, purified immunoglobulin

Synonym(s):

Anti-6330409F21Rik, Anti-FUBP2, Anti-FBP2, Anti-KH-type splicing regulatory protein, Anti-KHSRP, Anti-KSRP, Anti-MARTA1, Anti-MGC99676

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

KS-8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~82 kDa

species reactivity

rat, mouse, human, chicken, bovine, canine, hamster

concentration

~1.0 mg/mL

technique(s)

flow cytometry: 2-4 μg/test using HEK-293T cells
immunoblotting: 0.25-0.5 μg/mL using HEK-293T total cell extracts
immunocytochemistry: suitable
immunofluorescence: 2.5-5 μg/mL using HEK-293T cells

isotype

IgG1

UniProt accession no.

Q92945

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KHSRP(8570)

General description

Monoclonal Anti-KHSRP (mouse IgG1 isotype) is derived from the hybridoma KS-8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. K homology type-splicing regulatory protein (KHSRP) is an nucleic acid binding protein. It is localized in the cytoplasm and nucleus of the cell. It contains four K homology RNA-binding domains, two N- and C-terminals and protein interaction motifs. KHSRP gene is located on the human chromosome at 19p13.3.

Specificity

Monoclonal Anti- KHSRP recognizes human, cow, chicken, dog, hamster, rat and mouse KHSRP.

Immunogen

synthetic peptide corresponding to an internal sequence of human KHSRP

Application

Monoclonal Anti-KHSRP antibody produced in mouse may be used in:
  • immunoblotting
  • immunocytochemistry
  • flow cytometry
  • immunofluorescence

Biochem/physiol Actions

K homology type-splicing regulatory protein (KHSRP) acts as a transcription factor that affects RNA fates at multiple levels including mRNA decay and alternative pre-mRNA splicing. It is involved in rapid decay of AU-rich element (ARE)-containing mRNAs and maturation of microRNAs (miRNAs). KHSRP is significantly modulated in response to breast cancer type 1 (BRCA1) expression, suggesting this factor may contribute to the BRCA1-associated tumor phenotype.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
083M4807V

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BRCA1 modulates the expression of hnRNPA2B1 and KHSRP
Santarosa M, et al.
Cell Cycle, 9(23), 4666-4673 (2010)
Functional and molecular insights into KSRP function in mRNA decay
Briata P, et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1829(6-7), 689-694 (2013)
Mapping of theKHSRPGene to a Region of Conserved Synteny on Human Chromosome 19p13. 3 and Mouse Chromosome 17
Ring H Z, et al.
Genomics, 56(3), 350-352 (1999)

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