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SAB4200594

Sigma-Aldrich

Anti-APC (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-Adenomatous polyposis coli protein, Anti-BTPS2, Anti-DP2, Anti-DP2.5, Anti-DP3, Anti-FAP, Anti-GS
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~300 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells.
immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum.
indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells.

UniProt accession no.

P25054

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APC(324)

General description

Adenomatous Polyposis Coli (APC) is a tumor suppressor gene. The APC gene encodes a large multifunctional protein that has critical functions in cell migration, adhesion and proliferation. It is a shuttling nucleo-cytoplasmic protein expressed in various tissues and its expression in the colorectal epithelium contributes to its normal growth and differentiation.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human APC, conjugated to KLH. The corresponding sequence is identical in human APC isoform b and highly conserved (single amino acid substitution) in rat and mouse APC.

Application

Anti-APC (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Adenomatous Polyposis Coli (APC) has a key role in the proteasome-mediated degradation of β-catenin. APC mutations cause accumulation of β-catenin in the nucleus leading to activation of LEF-1 and/or TCF, and the induction of target genes such as the oncogene c-myc. It is involved in regulation of mitotic chromosome separation and stability.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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APC shuttling to the membrane, nucleus and beyond
Brocardo M and Henderson BR
Trends in Cell Biology, 18(12), 587-596 (2008)
APC, signal transduction and genetic instability in colorectal cancer
Fodde R, et al.
Nature Reviews. Cancer, 1(1), 55-55 (2001)
Adenomatous polyposis coli (APC): a multi-functional tumor suppressor gene
Aoki K and Taketo MM
Journal of Cell Science, 120(19), 3327-3335 (2007)

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