SAB4200572
Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal
clone NSE-P2, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-2-phospho-D-glycerate hydro-lyase, Monoclonal Anti-2-phospho-D-glycerate hydrolyase, Monoclonal Anti-Enolase 2 (gamma, neuronal), Monoclonal Anti-NSE, Monoclonal Anti-Neural enolase, Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse, Monoclonal Anti-gamma-enolase
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NSE-P2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~47 kDa
species reactivity
human, rat
concentration
~1 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.
western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ENO2(2026)
Related Categories
General description
Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31.
Specificity
Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE.
Immunogen
synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Application
Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
Biochem/physiol Actions
Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho-D-glycerate in the glycolytic pathway. NSE is a specific neurobiochemical marker of brain damage after traumatic brain injury, hypoxic encephalopathy and stroke. Moreover, NSE has been suggested to be a general circulating marker for neuroendocrine tumors. Interestingly, high levels of NSE were also reported in breast cancer upon exposure to the environmental pollutants arsenite and cadmium.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Linkage to peroxisome proliferator-activated receptor-gamma in SAMP1/YitFc mice and in human Crohn?s disease
Sugawara K, et al.
Gastroenterology, 128(2), 351-360 (2005)
The role of CEA, CYFRA21-1 and NSE in monitoring tumor response to Nivolumab in advanced non-small cell lung cancer (NSCLC) patients
DalBMG, et al.
Journal of Translational Medicine, 17(1), 74-74 (2019)
Neurological biomarkers in the perioperative period
Cata JP, et al.
British Journal of Anaesthesia, 107(6), 844-858 (2011)
Circulating biomarkers in gastroenteropancreatic neuroendocrine tumours
Oberg K
Endocrine-Related Cancer, 18(S1), S17-S25 (2011)
Arsenic, cadmium and neuron specific enolase (ENO2, gamma-enolase) expression in breast cancer
Soh MA, et al.
Cancer Cell International, 11(1), 41-41 (2011)
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