Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal

Monoclonal anti-neuron-specific enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Neuron specific enolase (NSE) belongs to the family of enolase enzymes. Enolases have three subunits (a, b, and g), which can combine to form five different isoenzymes: aa, ab, ag, bb, and gg. Enolase 1 (aa) is present in adipose tissue, kidney, liver, and spleen. Enolase 3 (bb) is muscle specific. Enolase 2 (ENO2), also known as neuron-specific enolase (NSE) is a cytoplasmic enzyme, that is released during cell destruction and has a half-life of approximately 30 hours in serum. This dimeric enzyme (gg) is found in neurons and in neuroendocrine cells. ENO2 gene is mapped to human chromosome 12p13.

Monoclonal Anti- Neuron-Specific Enolase (NSE) recognizes human, rat, and mouse NSE.

synthetic peptide corresponding to a sequence at the C-terminal region of human NSE.¹ The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal has been used in:

• immunofluorescence staining
• immunocytochemistry
• immunofluorescence microscopy
• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Enolase 2 (ENO2) participates in glycolysis by converting β-glycerophosphate into dihydroxyacetone phosphate. It serves as a neurobiochemical marker of brain damage after traumatic brain injury, anoxic encephalopathy, stroke, and cardiac arrest. NSE also acts as a circulating marker for neuroendocrine tumors. High levels of NSE is observed in breast cancer upon exposure to arsenite and cadmium.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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