SAB4200559
Anti-VAChT (C-terminal) antibody produced in rabbit
IgG fraction of antiserum
Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Anti-SLC18A3, Anti-Solute carrier family 18 member 3, Anti-VAT, Anti-vesicular acetylcholine transporter
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat, mouse, human
technique(s)
indirect immunofluorescence: 1:1,000-1:2,000 using PC12 cells treated with rat NGF.
western blot: 1:500-1:800 using extracts of mouse brain (S1 fraction).
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SLC18A3(6572)
mouse ... slc18a3(20508)
rat ... slc18a3(60422)
General description
Vesicular Acetylcholine Transporter (VAChT, SLC18A3) belongs to the solute carrier family 18 (SLC18) family of proton/neurotransmitter antiporters and shares a high degree of homology to vesicular monoamine transporters (VMATs). It is a 12-transmembrane domain protein. VAChT is expressed in all major cholinergic neurons in the central and peripheral nervous system and is mainly localized in small synaptic vesicles in cholinergic nerve terminals.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminus of human vesicular acetylcholine transporter (VAChT), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse VAChT (82% identity).
Application
Anti-VAChT (C-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence staining
- immunocytochemistry
- double immunolabeling
Biochem/physiol Actions
Vesicular Acetylcholine Transporter (VAChT), is required in cholinergic neurons for the selective transport of acetylcholine (ACh) into synaptic vesicles. It depends on the activity of a vacuolar-type H+ -ATPase to accumulate ACh in synaptic vesicles. Knockdown of VAChT in the hippocampus has been shown to cause behavior changes, learning deficits, specific deficits in spatial memory and long-term potentiation (LTP). VAChT provides a specific marker for cholinergic neurons, for the study of cholinergic transmission in experimental models of Alzheimer′s disease and other nervous system disorders. Cloning of the VAChT gene has revealed a single genetic locus encoding both VAChT and ChAT, providing a unique genomic arrangement suggesting that these genes may be co-regulated.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Vesicular acetylcholine transporter defect underlies devastating congenital myasthenia syndrome
Aran A, et al.
Neurology, 88(11), 1021-1028 (2017)
Long Li et al.
Frontiers in aging neuroscience, 13, 770948-770948 (2022-02-22)
To explore the effect of electro-acupuncture (EA) treatment on pattern separation and investigate the neural circuit mechanism involved in five familial mutations (5 × FAD) mice. Five familial mutations mice were treated with EA at Baihui (DU20) and Shenting (DU24)
Alzheimer's disease: targeting the cholinergic system
Ferreira-Vieira TH, et al.
Current Neuropharmacology, 14(1), 101-115 (2016)
B T Kipp et al.
Neuroscience, 473, 52-65 (2021-08-28)
During adolescence, heavy binge-like ethanol consumption can lead to frontocortical structural and functional impairments. These impairments are likely driven by adolescence being a critical time point for maturation of brain regions associated with higher-order cognitive functioning. Rodent models of heavy
Elimination of the vesicular acetylcholine transporter in the forebrain causes hyperactivity and deficits in spatial memory and long-term potentiation
Martyn AC, et al.
Proceedings of the National Academy of Sciences of the USA, 109(43), 17651-17656 (2012)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service