SAB4200545
Anti-Parvalbumin antibody, Mouse monoclonal
clone PARV-19, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-ALPHA-PARVALBUMIN, Monoclonal Anti-PALB1, Monoclonal Anti-PV, Monoclonal Anti-PVALB (includes EG:19293), Monoclonal Anti-Parv, Monoclonal Anti-Pva
UNSPSC Code:
12352203
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PARV-19, monoclonal
form
buffered aqueous solution
mol wt
antigen 12 kDa
species reactivity
rat, human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat cerebellum.
indirect immunofluorescence: 10-20 μg/mL using BLO-11 cells.
western blot: 1-2 μg/mL using mouse muscle extracts.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PVALB(5816)
rat ... PVALB(25269)
General description
Monoclonal Anti-Parvalbumin (mouse IgG1 isotype) is derived from the hybridoma PARV-19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified frog muscle parvalbumin. Parvalbumin (PV) is a member of the family of Ca2+-binding molecules. It carries three helix-loop-helix EF-hand Ca2+ binding motifs, with the two C-terminal motifs.
Specificity
Monoclonal Anti-Parvalbumin recognizes parvalbumin in a Ca2+ -ion dependent manner
Immunogen
purified frog muscle parvalbumin. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Application
Anti-Parvalbumin antibody, Mouse monoclonal has been used in:
- immunohistochemistry
- immunofluorescence staining
- immunolabeling
- to label brain slices for perineuronal net staining
- enzyme-linked immunosorbent assay (ELISA)
- immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Ca2+ participates in cell motility, release of neurotransmitters, membrane permeability, excitation−contraction of muscles, cell secretory procedures, and transmission of nerve impulses. Intracellular Ca2+ exchangers are essential to modulate these processes efficiently. Parvalbumin (PV), a calcium-binding protein, can regulate the intracellular calcium dynamics in neurons. Hence it may have an impact on facilitation and depression at synapses. Interestingly, PV deficiency alters neuronal activity, a key mechanism leading to epileptic seizures or Parkinson.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Rachel Schroeder et al.
Antioxidants & redox signaling, 35(7), 511-530 (2021-01-28)
Aims: Impaired embryonic cortical interneuron development from prenatal stress is linked to adult neuropsychiatric impairment, stemming in part from excessive generation of reactive oxygen species in the developing embryo. Unfortunately, there are no preventive medicines that mitigate the risk of
Syed Hasan Arif
BioEssays : news and reviews in molecular, cellular and developmental biology, 31(4), 410-421 (2009-03-11)
Parvalbumins (PVs) are acidic, intracellular Ca(2+)-binding proteins of low molecular weight. They are associated with several Ca(2+)-mediated cellular activities and physiological processes. It has been suggested that PV might function as a "Ca2+ shuttle" transporting Ca2+ from troponin-C (TnC) to
Stephanie J Lussier et al.
Developmental neurobiology, 76(10), 1078-1091 (2016-01-03)
Prenatal stress is associated with altered behavioral, cognitive, and psychiatric outcomes in offspring. Due to the importance of GABAergic systems in normal development and in psychiatric disorders, prenatal stress effects on these neurons have been investigated in animal models. Prenatal
Kyle A Sullivan et al.
International journal of molecular sciences, 24(14) (2023-07-29)
Over a third of patients with temporal lobe epilepsy (TLE) are not effectively treated with current anti-seizure drugs, spurring the development of gene therapies. The injection of adeno-associated viral vectors (AAV) into the brain has been shown to be a
Angela E Gonzalez et al.
Frontiers in cellular neuroscience, 16, 932391-932391 (2022-08-16)
Parvalbumin (PV)-positive cells are GABAergic fast-spiking interneurons that modulate the activity of pyramidal neurons in the medial prefrontal cortex (mPFC) and their output to brain areas associated with learning and memory. The majority of PV cells within the mPFC are
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