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Merck
CN

SAB4200506

Anti-ATG101 antibody, Mouse monoclonal

clone ATG101-15, purified from hybridoma cell culture

Synonym(s):

Anti-C12orf44, chromosome 12 open reading frame 44, PP894, Atg13-interacting protein, Anti-autophagy-related protein 101, Monoclonal Anti-ATG101 antibody produced in mouse

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
ATG101-15, monoclonal
Application:
WB
Citations:
5

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

ATG101-15, monoclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells overexpressing human ATG101.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... C12orf44(60673)

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General description

Monoclonal Anti-ATG101 (mouse IgG1 isotype) is derived from the hybridoma ATG101-15 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Atg101 is conserved in humans and several eukaryotes. It is not conserved in yeast Saccharomyces cerevisiae. ATG101 gene is located on the human chromosome at 12q13.13.

Immunogen

synthetic peptide corresponding to an internal sequence of human ATG101, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, canine and bovine ATG101.

Application

Monoclonal Anti-ATG101 antibody produced in mouse is suitable for use in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Anti- ATG101 recognizes human ATG101.
Atg101 is a mammalian autophagy-related 13 (Atg13) binding protein essential for autophagy. It is associated with the Unc-51 like autophagy activating kinase 1 (ULK1), Atg13, focal adhesion kinase (FAK) family kinase-interacting protein of 200 kDa (FIP200) complex via direct interaction with Atg13. Autophagy is an evolutionarily conserved pathway seen in all eukaryotic cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
092M4769V

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Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via In Silico Analysis.
Erzurumlu Y
Journal of Health Science, 11(1) (2020)
Zhanqin Zhang et al.
Cell death & disease, 12(2), 219-219 (2021-02-28)
At present, no systematic and in-depth study is available on the function and potential mechanisms of circular RNA in autophagy. This study aimed to screen the expression profiles of circRNA, miRNA, and mRNA of ovarian cancer cells induced by Torin
Structure of the human Atg13-Atg101 HORMA heterodimer: an interaction hub within the ULK1 complex
Qi S, et al.
Structure, 23(10), 1848-1857 (2015)
A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy
Mercer C A, et al.
Autophagy, 5(5), 649-662 (2009)
Atg101, a novel mammalian autophagy protein interacting with Atg13
Hosokawa N, et al.
Autophagy, 5(7), 973-979 (2009)

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