Anti-SND1 antibody, Mouse monoclonal

Monoclonal Anti-SND1 (mouse IgG1 isotype) is derived from the hybridoma SND1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. The Staphylococcal nuclease and tudor domain containing 1 (SND1) protein is highly conserved from yeast to human. It contains five repeated staphylococcal nuclease-like domains and a tudor-like domain, probably required for its interaction with nucleic acids and proteins, respectively. The SND1 protein is a constituent of the RNA-induced silencing complex (RISC). The SND1 gene is mapped to the human chromosome location 7q31.3.

Monoclonal Anti-SND1 recognizes human, mouse, rat, hamster and bovine SND1.

Synthetic peptide corresponding to an internal region of human SND1, conjugated to KLH. The corresponding sequence differs by a single amino acid in mouse and rat SND1.

Monoclonal Anti-SND1 antibody produced in mouse may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

The Staphylococcal nuclease and tudor domain containing 1 (SND1) protein is involved in the regulation of transcription. It also modulates the RNA interference (RNAi) function, RNA splicing, editing and stability. The SND1 protein promotes the degradation of hyper-edited inosine containing miRNA precursors. It modulates miRNA processing and expression through RNA editing by adenosine deaminase acting on RNA (ADAR). SND1 is up-regulated in human colon cancer, breast cancer and cancer cell lines. This protein promotes nucleic acid interaction along with protein-protein interactions. It also interacts with transcription factors as a transcriptional co-activator of Epstein-Barr virus nuclear antigen 2 (EBNA2) and signal transducer and activator of transcription (STAT5 and STAT6).

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.