SAB4200452
Anti-ADFP / PLIN2 antibody, Mouse monoclonal
clone ADFP-5, purified from hybridoma cell culture
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Synonym(s):
Adipophilin, Plin2 Antibody, Plin2 Antibody - Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse, Anti-ADRP, Anti-Perilipin 2, Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
ADFP-5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~48 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using human HepG2 or SH-SY5Y cells
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human ADFP
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PLIN2(123)
Related Categories
General description
Monoclonal Anti-ADFP/PLIN2 (mouse IgG1 isotype) is derived from the hybridoma ADFP-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide.
Perilipin 2 (PLIN2) is a 50 kDa major protein belonging to perilipin family. It is present on lipid droplets found in the cytoplasm of variety of cells as hepatocytes, adipocytes, muscle cells, mammary epithelial cells, fibroblasts, endothelial cells and macrophages. PLIN2 gene, spanning 14 kb, with eight exons, is located on human chromosome 9p22.1.
Specificity
Monoclonal Anti-ADFP/PLIN2 recognizes human ADFP/PLIN2.
Immunogen
a synthetic peptide corresponding to a sequence at the C-terminus of human PLIN2, conjugated to KLH.
Application
Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
PLIN2 may serve as a biomarker of lipid accumulation in diverse cell types and diseases.
Perilipin 2 (PLIN2) plays a vital role in uptake and storage of lipids as triacylglycerols in lipid droplets in skeletal muscle. PLIN2 is also involved in the regulation of lipid metabolism. High levels of PLIN2 is beneficial for the prevention of insulin resistance. Overexpression of PLIN2 is associated with clear cell renal cell carcinoma (CC-RCC).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of beta-oxidation
Larigauderie G, et al.
FEBS Journal, 273(15), 3498-3510 (2006)
Perilipin 2 improves insulin sensitivity in skeletal muscle despite elevated intramuscular lipid levels
Bosma M, et al.
Diabetes, DB_111402-DB_111402 (2012)
Overexpression of PLIN2 is a prognostic marker and attenuates tumor progression in clear cell renal cell carcinoma
Cao Q, et al.
International Journal of Oncology, 53(1), 137-147 (2018)
Identification and validation of a 44-gene expression signature for the classification of renal cell carcinomas
Wang Q, et al.
Journal of Experimental & Clinical Cancer Research, 36(1), 176-176 (2017)
Maria Conte et al.
PloS one, 8(8), e73709-e73709 (2013-08-27)
Human aging is associated with a progressive loss of muscle mass and strength and a concomitant fat accumulation in form of inter-muscular adipose tissue, causing skeletal muscle function decline and immobilization. Fat accumulation can also occur as intra-muscular triglycerides (IMTG)
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